Description  
        The 2xTaq PCR Master is an optimized ready-to-use PCR mixture of Taq DNA Polymerase, PCR buffer, MgCl2 and dNTPs. The 2xTaq PCR Master contains all components for PCR, except DNA template and primers. The Taq DNA has no 3’→5’exonuclease activity, so it is suitable to amplification of DNA fragment less than 5Kb, and the PCR product has an “A” base at the 3’terminal, thus it is very convenient to clone to T vector.
Package
        2xTaq PCR Master 1mL，40 runs
Conponent
        Taq DNA polymerase（2.5U/25ul）;
        4 dNTPs（each 0.4mM）;
        Mg²⁺（4mM）.
Protocol
        1、All solutions should be thawed on ice, gently vortexed and briefly centrifuged. Add in a thin walled PCR tube on ice, for a total 50μl reaction volume，each tube 25ul, then store at -20℃，avoiding frozen-thawn repeatedly；
        2、For 25ul 2xTaq PCR Master, add forward primer, reverse primer and template DNA, then add water (nuclease-free) to total volume 50ul；
        3、Place samples in a thermocycler and start the PCR program；
        4、Agarose gel electrophoresis analysis of PCR product.
Storage
    Store at -20℃.